RESULTS The first noticeable result was at first disappointing but turned around later in the trail. At first the rsaL mutation did not have any effect on the production of 30C12-HSL nor on lasl transcription, but it began to appear later in the logarithmic phase of propagation at which time the 30C12-HSL production along with Plasl activity reached homoeostasis in the wild strain while continuing to increase rsaL in the mutant

Molecular Basis Glanzmann Thrombasthenia An investigation of the molecular basis of Glanzmann Thrombasthenia using Polymerase Chain Reaction (PCR) The objective of this project is to investigate the molecular basis of Glanzmann Thrombasthenia (GT) using polymerase chain reaction. There have been many mutations discovered in GT patients over the years in many studies. Thus using PCR to genotype patients is one of the most effective ways of discerning the genetic basis of the

Expression Profiling of a Novel Protein A new transcription unit was discovered while working with transfected murine BAC clones, because a novel spot appeared on a 2-dimensional protein gel. Through a process of expression subcloning from the BAC clone, the transcription unit that generated the novel peptide was located. This finding was back validated by sequencing the protein contained in the 2-D gel piece using N-terminal Edman degradation and mass spectroscopy

Nanomachines The Science of molecular size machines and its engineering designs and constructions until late 1980s were not considered practicable. Nanotechnology, according to the leading exponents of that time were neither feasible nor viable, due to the fact of total structural difference of the constituent of nano-molecular device i.e. Atoms from the mechanical objects of every day life. The essential components of engineering mechanics i.e. cogwheels, gears or motors could not

laboratory-based practical work undertaken in this module was in relation to a case study of Systemic Lupus Erythematosus, SLE. SLE is a connective tissue disorder, which is autoimmune in nature. This disease affects multiple organs and its clinical manifestation is based on its severity and the organ involved. The pathogenesis of this disease is based on antigen-antibody complexes that circulate in the blood and deposit in the smaller blood

The failure of human hamster fusion in the presence of anti-human izumo antibody clearly showed that Human izumo protein is essential for fertilization. This study however has raised some new questions. The difference in fertilization capacities between wild type and Izumo +/- type was not clearly discussed. Also, the fact that ICSI of izumo -/- produces twice as many litters as did the izumo +/- type implicates the possible role