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Substance in Green Tea Inhibits Inflammatory Breast Cancer Cells
Green Tea & Inflammatory Breast Cancer Cells
What is the overall hypothesis of this experiment?
Consumption of epigallocatechin-3 gallate (EGCG) in green tea will reduce the distinct growth and dissemination properties of Inflammatory Breast Cancer (IBC) cells in culture and on tumor growth in an orthotopic mouse model, as mwasured by reduced growth, invasive phenotype, and survival of SMU-149 and SUM-190 IBC cells, and the ability of the IBC cells to stimulate in vitro lymphangiogenesis.
In the Materials and Methods section of the paper, the authors discuss the cell culture and treatment conditions. What was the control group treated with in this section (make sure to look only at the Cell Culture and Treatment section of the paper)? Why?
The control group was treated with carrier dimethyl sulfoxide (DMSO) equivalent to the highest does of EGCG used, which is calculated at DMSO = 0 ?g / ml EGCG. The DMSO chemical may be used to dissolve other chemicals or drugs that are otherwise difficult to dissolve in water. This step makes it easier to test substances in animal experiments or in cells that are growing in Petri dishes. When DMSO is used in this way, the experimental...
In cancer research, DMSO is used to help mature cancer cells or to differentiate noncancerous cells from cancerous cells through the capacity of DMSO to acquire certain characteristics of noncancerous cells.
3. Again, looking only at the Cell Cuture and Treatment section, what was the experimental group treated with? Why?
The experimental group was treated with doses of EGCG of 5, 10, 20, 40, 60, 80, or 160
g / ml, which correspond to 10.9, 21.8, 43.6, 87.3, 130.9, 174.5, and 349.0 ?g / ml, respectively. Essentially, this step represents the experimental condition, which is designed to test the effectiveness of EGCG on IBC cells.
4. In the Results section of the paper, the authors clearly summary their multiple findings. In the "EGCG Reduces Growth of Pre-existing Tumors Derived from SUM-149 Stem-like Cells" results sub-section, the authors report a specific finding. What specific result do the researchers report?
A statistically significant decrease in tumor size was detected in EGCG vs. The control populations at day 32. Indeed, the reduction in tumor size was observed to be 28 + / - 6.5% in animals treated by EGCG.…
Reference
Mineva et al. (2013). Epigallocatechin-3-gallate inhibits stem-like inflammatory breast cancer cells. PLoS ONE, 8(9): e73464. DOI:10.1371/journal.pone.0073464
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