Use of Embryonic Stem Cells to Cure Disease Research Paper

Download this Research Paper in word format (.doc)

Note: Sample below may appear distorted but all corresponding word document files contain proper formatting

Excerpt from Research Paper:

Embryonic Stem Cells to Cure Disease

Embryonic Stem Cell

Derivation of Human Embryonic Stem Cells

Generation of Cardiomyocytes from Human Embryonic Stem Cells

Purified Population of Cardiomyocytes

Use of Transgenes in Differentiated Cardiomyocytes

Use of Human Embryonic Stem Cells for Heart Conditions

Neurological Disorders and Use of Human Embryonic Stem Cells

Parkinson's Disease

Stroke

Huntington's disease

Amyotrophic Lateral Sclerosis

Human Embryonic Stem Cells for the Generation of Functional Hepatic Cells

Ethical Considerations of Using Human Embryonic Stem Cells

Social Oppression

Value of the Embryo

Pluripotent stem cell cultures were isolated in 1981 by Evans and Kaufman from mouse blastocysts. It was found that these cells were capable of self-renewal having a long-term capacity to remain undifferentiated in certain provided culture conditions. Studies have highlighted the basic difference between stem cells and embryonic stem cells. Embryonic stem cells have the potential to differentiate into three germ layers. These cells have an additional capacity to proliferate in culture conditions in an undifferentiated state plus these cells usually disappear after differentiating into germ layers. For clinical purposes, origin of human embryonic stem cell is pre-implantation embryo. The stem cell lines have been derived from inner cell mass of human blastocysts that are produced by in-vitro fertilization. The studies have shown that human embryonic stem cells have the properties of embryonic stem cells (Cai et al. 2007, p. 1229). The properties include derivation from pre-implantation embryo, prolonged proliferation in the culture in an undifferentiated state and a capacity to form three germ layers. In addition to this, it has been seen that human embryonic stem cells can maintain diploid karyotype and an expression of higher telomerase activity when cells are kept in cultures for longer periods.

There are two main properties of embryonic stem cells, indefinite cell renewal and an ability to differentiate into one or more cell types. Successful studies and applications of murine embryonic stem cell research have paved the way to study more about important applications of human embryonic stem cells. Several tissues in the human body depend on a pool of adult or somatic stem cells for maintenance. The tissues include hematopoietic system, skin, gut and some parts of the central nervous system. Studies have shown that the depletion of stem cell pools can lead to many diseases that include leukemia, lymphoma, and certain genetic defects. Other kinds of diseases involve tissue destruction where these tissues are unable to be revived by the stem cell pools. These diseases and conditions include Type 1 diabetes that occurs because of auto-immune destruction of pancreatic beta cells and liver failure occurring because of liver cirrhosis either because of toxins or certain infectious agents (Zou et al. 2009, p. 98). Main way to treat these diseases is replacement of stem cell pools in the body. Reports and studies have highlighted success of bone marrow transplants and direct organ transplants. Human embryonic stem cells can be triggered to differentiate into adult stem cells in order to replace the damaged stem cell pool in the body clinically in order to regenerate damaged or diseased tissues and organs (Lerou, and Daley, 2005, p. 321). Organ transplantation has been lesser appreciated as compared to replenishment of stem cells based on an immune barrier, where immune-suppression becomes necessary to prevent graft rejection (Gepstein, 2002, p. 869).

For proper clinical and medical usage of embryonic stem cells, it is important that developmental pathways of tissues within an embryo are studied and understood. Many kinds of embryonic stem cells have been characterized including insulin secreting cells, neural tissue, cardiomyocytes, endothelial cells, hematopoietic cells, hepatocytes and osteoblasts. Thereby stem cells can be used clinically in order to treat medical conditions in these tissues and cells (Lindvall, & Kokaia, 2006, p. 1095).

Derivation of Human Embryonic Stem Cells

It has been reported that cells in mammalian embryo have the capacity to regenerate into any tissue type in the body. This property is termed as pluripotency. After the occurrence of fertilization, at the stage of blastocyst, formation of hollow sphere of cells takes place that has an outer cell layer and an inner cell mass. The outer cellular layer develops into trophectoderm giving rise to placenta and other tissues. All the other tissues in the body are developed by the inner cell mass (Cai et al. 2007, p. 1231).

Generation of Cardiomyocytes from Human Embryonic Stem Cells

For clinical usage, many protocols have been used in order to trigger differentiation of human embryonic stem cells to specialized cardiomyocytes. Studies have shown that 5-aza-20-deoxycytodine has a great enhancing effect on differentiation of human embryonic stem cells towards cardiomyocytes (Mummery et al. 2002, p. 2734). However in these cases, the obtained cardiomyocytes are immature and have properties and functions related to fetal cardiomyocytes. Thereby there is a need that better protocols are developed that can help in the development and differentiation of human embryonic stem cells towards specialized cardiomyocytes rather than fetal or immature cardiomyocytes (Stojkovic et al. 2004, p. 260).

Purified Population of Cardiomyocytes

Second main fact highlighted in the studies is that in order to attain purified population of engrafted heart cells, it is important that protocols are followed that can generate purified population of heart cells. For clinical usage, purified cell population is used rather than mixed population for best results. For example, in certain clinical and medical conditions, it is important that only specific kinds of cardiomyocytes are used rather than the mixed populations. In the case of myocardial infarction and chronic heart failure, ventricular cardiomyocytes are needed, not sinus-nodal type because sinus-nodal type are more arrythmogenic and are reported to have caused morbidity in patients (Bhattacharya et al. 2004, p. 2959).

Use of Transgenes in Differentiated Cardiomyocytes

Human embryonic stem cells can generate differentiated heart cells when specific culture conditions are provided that can be used for clinical purposes that include heart failure and myocardial infarctions. In some cases, the previous studies have reported the usage of transgenes. Reports and studies have highlighted that transgenes can be a source of mutagenesis in the cells affecting the functions of cells in a negative way. The only advantage highlighted in the case of transgenic approach is that it can help in highlighting developmental pathways helping the scientists in learning about the lab culture conditions appropriate for the development of cardiomyocytes without any reliance on transgenes (Stojkovic et al. 2004, p. 263).

Use of Human Embryonic Stem Cells for Heart Conditions

In the western world, one of the main heart diseases being the main reason of mortality includes ischemic heart disease. Within the heart, irreversible cell damage is triggered based on oxygen deprivation and irreversible cell damage can cause heart cell death. The damaged cells are thereby in a need to be replaced by newer cells provided by cardiomyocytes transplantation. One of the main advantages in these cases is that permanent damage can be slowed down as newer cells can replace newer ones (Cai et al. 2007, p. 1232).

Neurological Disorders and Use of Human Embryonic Stem Cells

Some of the most common neurological disorders include multiple sclerosis, Parkinson's disease, and stroke. These diseases and conditions are caused by the loss of neurons and glial cells. In recent era, it is being hoped that a large and an inexhaustible source of neurons and glia will be provided by the stem cells in order to ensure success of therapies of the highlighted medical conditions to trigger cell replacement and neuroprotection. For this purpose, embryonic stem cells or fetal or adult stem cells from adult or fetal central nervous system are considered as best suitable. For clinical applications of these cells, it is important that specific kinds of cells and neuroprotective molecules are used (Gepstein, 2002, p. 866).

Parkinson's disease

Parkinson's disease is characterized by the gradual loss of nigrostriatal dopamine-containing neurons but in some cases it has been reported that there is a loss in non-dopaminergic neurons as well. Main symptoms of Parkinson's disease include lesser movement, rigidity, tremors, and an increased instability in postural movements. Some of the main therapies that are being used in the modern days include oral administration of L-Dopa along with dopamine receptor agonists (Lindvall and Kokaia, 2006, p. 1095). Another therapy includes the deep brain stimulation of the subthalamic nucleus. These treatments have been seen to be effective for some symptoms. There are some associated disadvantages in the case of these treatments and these include an inability to control the progression of the disease. Thereby there is a need to find an alternative that can help stop the progression of the disease and can cure the disease rather than treating some symptoms. Significant improvements in mobility and long lasting disease cure are what needs to be targeted by the help of stem cells (Bhattacharya et al. 2004, p. 2957).

Many clinical trials have been conducted where fetal dopaminergic neurons have been transplanted replacing the damaged dopaminergic neurons showing major and long lasting improvements in the patients. Thereby most promising results have been shown by embryonic stem cells…[continue]

Cite This Research Paper:

"Use Of Embryonic Stem Cells To Cure Disease" (2011, September 24) Retrieved December 9, 2016, from http://www.paperdue.com/essay/use-of-embryonic-stem-cells-to-cure-disease-45728

"Use Of Embryonic Stem Cells To Cure Disease" 24 September 2011. Web.9 December. 2016. <http://www.paperdue.com/essay/use-of-embryonic-stem-cells-to-cure-disease-45728>

"Use Of Embryonic Stem Cells To Cure Disease", 24 September 2011, Accessed.9 December. 2016, http://www.paperdue.com/essay/use-of-embryonic-stem-cells-to-cure-disease-45728

Other Documents Pertaining To This Topic

  • Moral Issues Relating to the Therapeutic Use of Embryonic Stem Cells...

    Therapeutic Use of Embryonic Stem Cells in Humans Moral issues relating to the therapeutic use of embrionic stem cells in humans Moral issues relating to the therapeutic use of embryonic stem cells in humans The inception of the idea of research of stem cells became plausible in several decades, in the past. Additionally, the research caused an ensuing countless debates to accompany the issue. In considering the research on the stem cell, several

  • Embryonic Stem Cell Research the Use of

    Embryonic Stem Cell Research The use of human embryonic stem cells in scientific research has held great promise for some but this research has also produced powerful objections from others. Indeed, there is a profound if sometimes vehemently expressed moral argument that emerges from embryonic stem cell research. The principal objections to the use of these stem cells has come from evangelicals, conservative Christians and others who equate using embryonic stem

  • Embryonic Stem Cell Research in

    Conclusion The research showed that one of the most important, but hotly debated, issues in biomedicine in recent years has been embryonic stem cell research. The fundamental questions involved in this debate relate to the status of human embryos and whether any type of research that involves the destruction of a potential human being is morally and legally defensible. The research also showed, though, that the vast majority of the stem

  • Embryonic Stem Cell Research

    In avoiding the current controversy on the morality of embryonic stem cell research, researchers and doctors have resorted to other options (Dobson 2004, National Review 2004). Substitutes like adult stem cells and somatic cell nuclear transfer from placental or umbilical cord stem cells of newborns. Adult stem cells, however, were found to be nearly not as malleable as human embryonic stem cells or those acquired through somatic cell nuclear

  • Stem Cell Research Embryonic Stem Cell

    (Condic, 31) Scientists visualize immeasurable value in the application of embryonic stem cell research to comprehend human growth and the development and healing of ailments. More than 100 million Americans are ailing from the diseases that subsequently might be dealt more successfully or even cured with embryonic stem cell procedure. Majority of the researchers consider stem cell research as having large prospects for healing human ailments ever since the

  • Genetics Embryonic Stem Cells Embryonic

    Provided with the right mixture of signals, embryonic stem cells can develop into mature cells that can function as neurons, muscles, bone, blood along with other needed types of cells. Stem cells that have this flexibility are portrayed as pluripotent, in order to indicate their high potential to differentiate into a wide variety of cell types. A second attribute of embryonic stem cells is that they have the ability

  • Human Embryonic Stem Cell Research

    However, unlike embryonic stem cell, adult stem cell cannot be as easily controlled. Conclusion Because of its complicated theory and controversy, many people do not know much about stem cell research. Some people do not even know that there are two different types of stem cells: embryonic stem cells and adult stem cells. While many people are still in favor of embryonic stem cell research, the alternative -- adult stem cells


Read Full Research Paper
Copyright 2016 . All Rights Reserved