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The question was what type of bacteria was being sequenced. The steps used were: 1) Extraction of genomic DNA, 2) Amplification of genetic region, 3) Verification, 4) Clean PCR Products, 5) Quantify DNA concentration, 6) Cycle the sequence, and, 7) Precipitate cycle-sequenced products for analysis. In this case, a virtual laboratory was used, with the user being guided to the appropriate tools and equipment (Virtual Bacterial Identification).
Results
Accession
Description
Max score
Total score
Query coverage
E value
Max ident gi|39295|Z11684.1
R.henselae 16S rRNA gene
0.0
gi|6626180|AF214556.1
Bartonella henselae 16S ribosomal RNA, partial sequence
0.0
99%
Discussion- The BLAST search displays the matching sequences in the database in descending order of the degree of the match. Most of the top scorers are either Bartonella henselae or Rochalimaea henselae. The latter is an older name for this species. Bartonella species are demanding in their nutritional requirements for culture; as a result, normal culturing techniques do not yield vigorously growing bacterial colonies.
Bartonella henselae Various species of Bartonella that are pathogenic to humans are transmitted via a vector, or directly from an animal reservoir. For...
bacilliformis via sandflies causes Oroya fever; B. quintana via body lice causes trench fever; and B. henselae via cats causes cat scratch disease (CSD). CSD typically manifests as swellings of the lymph glands, possibly with skin lesions at the site of inoculation and possibly accompanied by fever, fatigue, and other symptoms. Immunocompromised patients may be particularly susceptible and can develop a different disease, bacillary angiomatosis, as a result of infection by B. henselae or B. Quintana (Virtual Bacteria Identification).
REFERENCES
Nobel, I. "Secret of Life Discovery Turns 50." 27 Feburary 2007. BBC News. August 2010 .
"Virtual Bacterial Identification." January 2010. The Virtual Bacterial ID Lab. August 2010 .
Walker and Jones. Genes and DNA. Kingfisher, OK: Kingfisher Press, 2003.
APPENDIX A -- Genetic Map of
Search:
Query ID: lcl|19505
Accession
Description
Max score
Total score
Query coverage
E value
Max ident gi|39295|Z11684.1
R.henselae 16S rRNA gene
0.0
gi|6626180|AF214556.1
Bartonella henselae 16S ribosomal RNA, partial sequence
0.0
99%
gi|2828303|AJ223779.1
Bartonella henselae 16S rRNA gene, isolate FR96/BK38
REFERENCES
Nobel, I. "Secret of Life Discovery Turns 50." 27 Feburary 2007. BBC News. August 2010 <http://news.bbc.co.uk/2/hi/science/nature/2804545.stm>.
"Virtual Bacterial Identification." January 2010. The Virtual Bacterial ID Lab. August 2010 <http://www.hhmi.org/biointeractive/vlabs/bacterial_id/>.
Walker and Jones. Genes and DNA. Kingfisher, OK: Kingfisher Press, 2003.
APPENDIX A -- Genetic Map of
The purified DNA is then prepared using a PCR like procedure that is described in detail by Innis and then can be automatically sequenced using standard methods (Hirashi). The resulting DNA sequence can then be entered into the NCBI database to search for a bacterial match. The database can be found at the following web address: http://blast.ncbi.nlm.nih.gov/Blast.cgi. Results Using PCR and DNA sequencing techniques it was determined that the bacteria isolated
Nonspecific staining in both the control and experimental groups rendered any possible findings virtually invisible, but ultimately the opportunity to participate in the research process from hypothesis through to data presentation was a tremendous opportunity for me, and I highly value the experience. My taste for research had only just been awoken by my experiences in my third year as an undergraduate, and after completing my degree I signed on
Ian Wimut and Keith Campbell could effectively clone two sheeps named Megan and Morag in July 1995 from the differentiated embryo cells. (History of Cloning) Dolly originated on July 5, 1996 as the first organism ever to be cloned from adult cells. Following the announcements for creation of Dolly by Ian Wilmut, an extensive debate on human cloning ethics emerged and that led President Clinton to propose for a five-year
Conclusion Despite the depressing figures embodied in the quote introducing this thesis, that: "The overall cure rate for AML…is between 40 and 45%" (Belson, Kingsley, and Holmes, para. 6), data/information related during the next chapter, the Literature Review, will contain a semblance of hope. Hope for the potential development of significant improvement of therapies for AML, the researcher projects, albeit, depends on continuing studies such as the three noted in/by this
123). In this study, Martinez-Contreras and her associates report the results of recent research that has provided additional evidence concerning the function of these proteins in precursor-messenger RNA (pre-mRNA) splicing (2007). The splicing repression can function in two discrete ways in heterogeneous nuclear RNP proteins; the first way is by antagonizing the recognition of splice sites directly and the second way is through interference with the binding of proteins that